keratinocyte growth medium 3  (PromoCell)

Journal: Redox Biology

Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

doi: 10.1016/j.redox.2026.104069

Figure Lengend Snippet: Keratinocytes cultured on modified collagen type IV show oxidative stress and reduced MMP levels. A Scatter plots show gene expression levels of IL1A and HMOX1 relative to B2M in keratinocytes cultured on HNE or OxPAPC modified collagen type IV. Primary keratinocytes from two donors (f38y, m27y) were grown in quadruplicates for up to 72 h, n = 8. B Heatmap of log 2 -transformed relative expression levels normalized to the corresponding control. Transcriptional levels of senescence, inflammation, and keratinocyte differentiation markers were assessed. Colors represent expression changes (blue = downregulation, red = upregulation). Statistical significance was determined by one-way ANOVA and is indicated by asterisks (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001) with p-values shown for z values > 1.

Article Snippet: After gelation at 37 °C in a humidified atmosphere for 3 h in the absence of CO 2 , gels were equilibrated in Keratinocyte Growth Medium (KGM2, PromoCell, Germany) and placed into an incubator with 5% CO 2 .

Techniques: Cell Culture, Modification, Gene Expression, Transformation Assay, Expressing, Control

Journal: Redox Biology

Article Title: Modification of the dermal matrix by senescence associated lipids and its functional consequence

doi: 10.1016/j.redox.2026.104069

Figure Lengend Snippet: Skin equivalents with modified collagen show disturbed differentiation and early senescence. A Hematoxylin & Eosin staining of skin equivalents. Collagen was modified, fibroblasts from different donors (f34y, f42y, m25y) were seeded into the matrix, and keratinocytes (f49y, f35y, f30y) on top; n = 6. SE were cultured at the air-liquid interface for 5 days to allow differentiation. B Quantification of nuclei in stratum corneum, indicating parakeratosis, n = 10. Bar graph shows mean ± SD; significant differences determined by one-way ANOVA (* p < 0.05; ** p < 0.01; *** p < 0.005; **** p < 0.001). C Representative images of SE stained with anti -KRT10, anti -KRT14, and Hoechst nuclear counterstaining. D Boxplot showing quantification of epidermal thickness, n = 4. Data are represented as median ± interquartile range; significance was determined by one-way ANOVA. E Protein expression of cell cycle inhibition marker P16 and housekeeping marker TUBULIN from the epidermis of the SE. F Bar chart showing quantification of P16 vol intensity normalized to TUBULIN with mean ± SD, n = 4, differences determined by one-way ANOVA. G Scatter plots showing expression levels of LMNB1, CDKN1A , and HSPA1A relative to B2M from epidermis and dermis of SE, n = 6. Asterisks represent significant differences determined by one-way ANOVA. H Confocal images of SE with anti -LMNB1 immunostaining and Hoechst nuclear counterstaining. I Boxplot showing quantification of LaminB1 mean signal intensity per pixel, measured in a virtual cross section through the epidermal cells, n = 4, significance determined by one-way ANOVA. J Confocal images of SE with anti -γH2AX immunostaining and Hoechst counterstaining. K Bar chart showing quantification of γH2AX positive nuclei by tissue cytometry analysis of the entire tissue section, mean ± SD, n = 3. Significant differences (* p < 0.05; ** p < 0.01) determined by Student's t - test. Scale bars: A = 50 μm; C = 100 μm; H, J = 20 μm.

Article Snippet: After gelation at 37 °C in a humidified atmosphere for 3 h in the absence of CO 2 , gels were equilibrated in Keratinocyte Growth Medium (KGM2, PromoCell, Germany) and placed into an incubator with 5% CO 2 .

Techniques: Modification, Staining, Cell Culture, Expressing, Inhibition, Marker, Immunostaining, Cytometry